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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene continues to be noticed in lots of tumors, which ends up in chromosome instability, and thus might contribute to tumorigenesis. This protein was located to affiliate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a critical function in marketing cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been explained. Two additional splice variants ended up reported but detailed nucleotide sequence information and facts is not however readily available.

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Blocking peptides are peptides that bind specially into the target antibody and block antibody binding. These peptide commonly contains the epitope acknowledged because of the antibody.

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This information outlines more feasible optimization of mobile and immunoassay parameters to guarantee the absolute best final results are...

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This guidebook outlines even more attainable optimization of mobile and immunoassay parameters to be sure the absolute best effects are...

Antibodies sure to the blocking peptide no more bind to your epitope to the focus on protein. By comparing the staining from your blocked antibody compared to the antibody on your own, one can see which staining is specific.

Concentrate on Information and facts Cyclin E belongs into the remarkably conserved cyclin relatives, whose associates are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute into the temporal coordination of each and every mitotic function. Cyclin E forms a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S section boundary which is degraded as cells progress as a result of S period.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...

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